黄颖，医学博士，博士
LANGUAGES
研究项目和附属机构
研究兴趣
研究抽象
Oncogenic signaling pathways in cellular transformation and apoptosis
癌变是一个多步骤的过程，包括原癌基因的激活和肿瘤抑制基因的失活. Gain-of-function mutations in oncogenes are frequently detected in human cancers. 癌基因的激活在癌症的发展和改变细胞对抗癌药物的敏感性方面起着重要作用. 我实验室的研究主要集中在以下几个方面:(1)癌细胞增殖中的细胞激酶信号传导, survival and death; (2) Studying the function and regulation of tumor suppressor RASSF1A; (3) Investigating the role of a novel family of GTPases, RBEL1A, B, C and D in regulation of cell death and proliferation; (4) Investigating the role of a novel growth suppresive lipase in cell growth control and cancer development; (5) Identification and development of novel anticancer agents using traditional Chinese medicines.
选择出版物:
Meltzer, S. J., Yin, J., Huang, Y.麦克丹尼尔，T. K.纽柯克，C., Iseri, O.Vogelstein, B. 和瑞索，J. Reduction to homozygosity involving p53 in esophageal cancers demonstrated by the polymerase chain reaction. Proc. Natl. Acad. Sci. 中国生物医学工程学报，1991,18 (3):491 -498.
Boynton, R. F., Huang, Y., Blount, P. L., Reid, B.J., Raskind, W. H., Haggitt, R. C.纽柯克，C., Resau, J. H., Yin, J.丹尼尔，T. 梅尔泽，S. J. Frequent loss of heterozygosity at the retinoblastoma locus in human esophageal cancers. Cancer Res., 1991, 51: 5766-5769.
McDaniel, T.K., Huang, Y.， Needleman, S.W. 梅尔泽，S. J. Direct labeling of polymerase chain reaction products using Klenow fragment. 生物医学工程学报，1997,11 (1):164- 164.
McDaniel, T.K.碳，D.高桥，T.Chumakov, P., Chang, E.H.皮罗洛，K.F., Yin. J., Huang, Y.梅尔泽，S. J. Polymorphism in intron 6 of p53 detected by digesting PCR products with MSP I. 核酸类., 1991, 19: 4796.
Boynton, R. F., Blount, P. L., Yin, J., Brown, V., Huang, Y., Tong, Y.麦克丹尼尔，T.纽柯克，C., Resau, J., Raskind, W. H., Haggitt, R. C., Reid, B. 梅尔泽，S. J. Loss of heterozygosity involving the APC and MCC genetic loci occurs in majority of human esophageal cancers. Proc. Natl. Acad. Sci. 中国生物医学工程学报，1992,19 (3):385- 388.
格林沃尔德,B., Huang, Y., Baum. R.梅尔泽，S. J. Barrett's carcinoma in a 25-year-old man with point mutation of the p53 tumor suppressor gene. Int. J. 中华肿瘤杂志，1992,1:271-275.
格林沃尔德,B. D., Harpaz, N., Yin, J., Huang, Y., Tong, Y., Brown, V. L.麦克丹尼尔，T.纽柯克，C., Resau, J. H. 梅尔泽，S. J. Loss of heterozygosity affecting the p53, Rb, and mcc/apc tumor suppressor gene loci in dysplastic and cancerous ulcerative colitis. Cancer Res., 1992, 52:741-745.
Huang, Y.博因顿，R. F., Blount, P. L.Silverstein, R., Yin, J., Tong, Y.纽柯克，C., Resau, J. H. Sridhara, R., Reid, B. J. 梅尔泽，S. J. Loss of heterozygosity involves multiple tumor suppressor genes in human esophageal cancers. Cancer Res., 1992, 52:6525-6530.
Huang, Y.梅尔泽，S. J., Yin, J., Tong, Y., Chang, E. H.西尔瓦斯塔瓦，S.麦克丹尼尔，T.博因顿，R. F., Zou, Z-Q. Altered mRNA and unique mutational profiles of p53 and Rb in human esophageal carcinomas. Cancer Res., 1993, 53:1889-1894.
Yin, J., Harpaz, N., Tong, Y., Huang, Y., Laurin, J.格林沃尔德，B. D.洪塔诺萨斯，M.纽克，C.梅尔泽，S. J. p53 point mutations in dysplastic and cancerous ulcerative colitis lesions. Gastroenterology, 1993, 104:1633-1639.
Blount, P. L.梅尔泽，S. J., Yin, J., Huang, Y., Krasna, M. J.里德，B. J. Clonal ordering of 17p and 5q allelic losses in Barrett's dysplasia and adenocarcinoma. Proc. Natl. Acad. Sci.[j] .中国生物医学工程学报，1993,9:322 - 325.
Huang, Y., Yin, J.梅尔泽，S. J. 在人类食管癌中，一种独特的p53基因内缺失，其两侧是短的直接重复序列，导致mRNA表达缺失. 中华肿瘤杂志，1994,15 (5):1653-1655.
Huang, Y., Saez, R., Chao, L., Santos, E.艾伦森，南卡罗来纳州. A和Chan. M-L. TC21基因的一个新的插入突变激活了它在人类平滑肌肉瘤细胞系中的转化活性. 中华肿瘤杂志，1997,11:1255-1260.
Huang, Y., Chan, A. M-L., Liu, Y., Wang, X.新罕布什尔州霍尔布鲁克市. J. 血清停药和依托泊苷通过不同途径诱导人肺癌A549细胞凋亡. 中国生物医学工程学报，1997,22 (2):591 - 596.
Wang, X.格罗斯，M., Huang, Y.纽约州霍尔布鲁克(Holbrook). J. p27/Kip1 over-expression causes apoptotic death of mammalian cells. 中华肿瘤杂志，1997,15 (5):2991-2997.
Sheikh, M.S., Burns, T.F., Huang, Y., Wu, G. S.阿蒙森，S., Brooks, K. S.小福纳斯., A. J. El-Deiry, W.S. 基因毒性应激和肿瘤坏死因子α对死亡受体KILLER/DR5基因表达的依赖性和非依赖性调控. Cancer Res., 1998, 588:1593-1598.
Sheikh, M. S.安提诺，M. J., Huang, Y. 还有小福纳斯., A. J. 紫外线照射诱导的细胞凋亡是通过不依赖配体的肿瘤坏死因子受体1激活介导的. 中华肿瘤杂志，1998,17 (5):555- 563.
Huang, Y., Sheikh, M. S., Fornace, A. J.新罕布什尔州霍尔布鲁克市. J. 丝氨酸蛋白酶抑制剂TPCK阻止紫杉醇诱导的人乳腺癌细胞死亡和c-Raf-1和bcl-2磷酸化. 中华肿瘤杂志，1999,18 (3):331 - 339.
Sheikh, M. S, Huang Y.费尔南德斯-萨拉斯，E. A.El-Deiry, W. S.阿蒙森，S., Yin J.梅尔泽，S. J.霍尔布鲁克. J.小福纳斯. A. J. 抗凋亡诱饵受体TRID/TRAIL-R3是p53调控的DNA损伤诱导基因，在胃肠道原发肿瘤中过表达. 中华肿瘤杂志，1999,18 (4):453 - 459.
Zhang Y., Huang Y., Rishi A., Sheikh, M. S., Shroot, B., Reichert U., Dawson M., Poirer, G.Fontana J. A. 新型类维甲酸介导细胞凋亡过程中p38和JNK/SAPK活化蛋白激酶途径的激活. Exp. Cell Res., 1999, 247: 233-40.
Sheikh, M.S.费尔南德斯-萨拉，E., Yu, M.侯赛因，A., Huang, Y.和小福纳斯., A. J. 编码翻译起始因子的哺乳动物基因毒性应激诱导cDNA的克隆和鉴定. J. Biol. Chem., 1999, 274: 16487-16493.
Huang, Y., Hutter, D., Liu, Y., Wang, X., Liu, Y., Sheikh, M. S.陈，A-M-L.新罕布什尔州霍尔布鲁克市.J. tgf
Huang, Y., He, Q.希尔曼，M.J., Rong, R., Sheikh, M.S. sulindac硫化钠诱导人结肠癌和前列腺癌细胞凋亡涉及死亡受体5和caspase 8依赖途径. Cancer Res., 2001, 61:6918-6924.
Rong, R., He, Q., Liu, Y., Sheikh, M.S. Huang, Y. TC21通过激活磷脂酰肌醇3-激酶/Akt和NF-kappa B信号通路介导转化和细胞存活. 中华肿瘤杂志，2002,21 (1):662 - 667.
He, Q., Lee, D.K., Rong, R., Yu, M., Luo, X., Klein, M., Huang, Y.侯赛因，A., Sheikh, M.S., 内质网钙池耗竭诱导的细胞凋亡与死亡受体5通路的激活有关. 中华肿瘤杂志，2002,21 (2):523 - 533.
He, Q., Rashid, A., Rong, R.希尔曼，M.J., Huang, Y., Sheikh, M.S. Death receptor 5 regulation during selenium-mediated apoptosis in human prostate cancer cells. 癌症生物学 & 中华医学杂志，2002,1 (3):379 - 379.
He, Q., Luo, X., Huang, Y. 谢赫先生.S. Apo2L/TRAIL差异调节舒林酸和COX-2选择性非甾体抗炎药对Bax缺陷细胞的凋亡作用. 中华肿瘤杂志，2002,21 (1):362 - 364.
He, Q.蒙塔巴诺.科科伦，C., Jin, W., Hunag, Y.谢赫，M.S. Bax缺乏对内质网钙池耗尽诱导细胞凋亡过程中死亡受体5和线粒体通路的影响. 中华肿瘤杂志，2003,22 (2):674- 679.
Luo, X., Huang, Y.谢赫，M. S. 一个受p53和紫外线辐射差异调控的新基因PDRG的克隆和表征. 中华肿瘤杂志，2003,22:747 - 757.
Sheikh, M.S., Huang, Y. Death receptor as targets of cancer therapeutics. 目前的癌症药物靶点. 2004, 4: 97-104.
Fahana, L., Dawson, M.I., Huang, Y., Zhang, Y., Rishi A.K.Reichert，美国.J .丰塔纳.A. 新型维甲酸CD437对乳腺和前列腺癌细胞中磷酸肌醇3-激酶和AKT激酶活性的抑制作用. 中华肿瘤杂志，2004,23:1874-1884.
He, Q., Huang, Y., Sheikh, M.S. Bax deficiency affects caspase-2 activation during ultraviolet irradiation-induced apoptosis. 中华肿瘤杂志，2004,23 (3):1321-1325.
He, Q., Huang, Y., Sheikh, M.S. 蛋白酶体抑制剂MG132上调死亡受体5并与Apo2L/TRAIL协同诱导bax精通和缺乏细胞凋亡. 中华肿瘤杂志，2004,23:2554-2558.
Rong, R. Jin, W., Zhang, J., Sheikh, M.S., Huang, Y. 肿瘤抑制因子RASSF1A是一种微管相关蛋白，可稳定微管并诱导G2/M细胞周期阻滞. 中华肿瘤杂志，2004,23:8216-8230.
Corcoran, C.A., He, Q., Huang, Y., and M. S. Sheikh. Cyclooxygenase-2 (COX-2) interacts with p53 and interferes with p53-dependent transcription and apoptosis. 中华肿瘤杂志，2005,24 (4):334 - 344.
Rong, R.蒙塔巴诺., Jin W., Zhang J., Garling M., Sheikh M.S., Huang Y. Oncogenic Ras-mediated downregulation of Gadd153/CHOP is required for Ras-induced cellular transformation. 中华肿瘤杂志，2005,24 (4):667 - 672.
Luo, X., He, Q., Huang, Y., Sheikh, M. S. PUMA的转录上调通过Bax激活调节内质网钙池耗尽诱导的细胞凋亡. Cell death and differentiation, Cell Death and Differentiation, 2005, 12:1310-8.
Luo, X., He, Q., Huang, Y., Sheikh, M.S. p53和DNA损伤下调基因PIQ的克隆和表征，该基因编码一种新的钙调素结合IQ基序，并在胃肠道癌症中上调. Cancer Res. 2005, 65:10725-10733.
Corcoran, C.A., Luo, X., He, Q., Jiang, C., Huang, Y., Sheikh, M.S. Genotoxic and endoplasmic reticulum stresses differentially regulates TRB3 expression. 癌症生物学 & 心理治疗，2005,4:1063-1067.
Rong, R., Jiang, LY.谢赫，MS.，黄，Y. 有丝分裂激酶Aurora-A磷酸化RASSF1A并调节RASSF1A介导的微管相互作用和m期细胞周期调节. Oncogene. 2007, 26: 7700-7708.
Montalbano J., Jin, W.谢赫，MS., Huang, Y. RBEL1是一种编码核细胞质Ras超家族gtp结合蛋白的新基因，在乳腺癌中过度表达. J. Biol Chem. 2007, 282:37640-37649.
He, Q., Luo, X., Jin, W., Huang, Y.拉玛娜·雷迪，m.s.V., Reddy, E. P. 谢赫先生.S. Celecoxib and a novel COX-2 inhibitor ON09310 upregulate Death Receptor 5 expression via GADD153/CHOP. 中华肿瘤杂志，2008,27 (2):556 - 560.
Corcoran, C.A., He, Q.Ponnusamy, S.奥格雷门，B., Huang, Y.谢赫，MS. 中性鞘磷脂酶-3是一种DNA损伤和非遗传毒性应激调节基因，在人类恶性肿瘤中不受调控. 分子癌症研究，2008; 6, 795-807. (封面文章)
He, Q., Shi, J., Jones, S., An, J., Liu, Y., Huang, Y., Sheikh, M.S. Smac deficiency affects endoplasmic reticulum stress-induced apoptosis in human colon cancer cells. Mol. Cell. Pharm. 2009, 1(1), 23-28.
Montalbano J., Lui, K.谢赫，MS., Huang, Y. 参与细胞生长调控的Ras超家族gtpase RBEL1亚家族的鉴定与表征. J. Biol Chem., 2009, 284, 18129-18142.
Corcoran, C.A.，蒙塔巴诺，J., Sun, H., He, Q., Huang, Y., Sheikh, M.S. 两种独立于环指结构域负调控p53的pih2泛素连接酶新亚型的鉴定和表征. J. Biol. Chem., 2009, 284, 21955-21970.
Shi, J., He, Q., An, J., Sun, H., Huang, Y., Sheikh, M.S. Sulindac sulfide differentially induces apoptosis in Smac-proficient and -deficient human colon cancer cells. Mol. Cell. Pham. 2009 1(2), 92-97.
Sun, H., Luo, X.，蒙塔巴诺，J., Jin, W., Shi, J., Sheikh, M.S., Huang, Y. DOC45, a novel DNA damage-regulated nucleocytoplasmic ATPase that is overexpressed in multiple human malignancies. 分子癌症研究, 2010, 8(1), 57-66.
He Q., Shi J., Shen X-L., An J., Sun H., Wang L., Hu Y-J., Sun Q., Fu L-C., Sheikh M.S., Huang Y. 双氢青蒿素上调死亡受体5表达并与TRAIL协同诱导人前列腺癌细胞凋亡. 癌症生物学 and Therapy, 2010, 9(10):819-24.
Jiang L., Luo X., Sun H., Sun Q., Sheikh M.S. Huang Y. PDRG1, a novel tumor marker for multiple malignancies that is selectively regulated by genotoxic stress. 癌症生物学 and Therapy, 2011, 11 (6).
Jiang L., Rong R., Sheikh M.S., Huang Y. Cul4A-DDB1 E3 ligase complex targets tumor suppressor RASSF1A degradation during mitosis. J. Biol. Chem. 2011, 286(9), 6971-6978.
An J., Shi J., He Q.，刘凯，刘勇., Huang Y., Sheikh MS. CHCM1/CHCHD6, a novel mitochondrial protein linked to regulation of mitofilin and mitochondrial cristae morphology. J. Biol. Chem. 2012, 287(10):7411-26
Sun H., Jiang L., Luo X., Jin W., He Q., An J., Lui K., Shi J., Rong R., Su W.卢切西C., Liu Y., Sheikh M.S., Huang Y. Potential tumor suppressive role of Monoglyceride Lipase in human colorectal cancer. 中华肿瘤杂志，2013,3(2):234-41.
Lui, K., An, J.，蒙塔巴诺，J., Shi, J.科科伦，C., He, Q., Sun, H., Sheikh, M.S., Huang, Y. Negative regulation of p53 by Ras superfamily protein RBEL1A. Journal of Cell Science, 2013, 126: (p11)2436-45.
Jiang, L., Rong, R.谢赫，MS., Huang, Y. Mitotic arrest by tumor suppressor RASSF1A is regulated via Chk1 phosphorylation. 分子癌症研究. 2014, 12(1):119-29.
Sun, Q., Liu, K., Shen, X., Jin, W., Jiang, L., Sheikh, M.S., Hu, Y., Huang, Y. Lappaol F, a novel anticancer agent isolated from plant Arctium Lappa L. 2013，分子癌症治疗. 2014, 13(1):49-59.
刘健，谢兆明，黄莹. Regulation of p53 oligomerization by Ras superfamily protein RBEL1A. Genes & Cancer. 2015, 6 (7-8): 1-10.
卢志平，谢赫女士，黄勇. Negative regulation of RNA-binding protein HuR by tumor suppressor ECRG2. 中华肿瘤杂志，2016,35(20):2565-73. doi: 10.1038/onc.2015.339. Epub 2015年10月5日.
Babbar M, Huang Y, An J, Landas SK, Sheikh MS. CHTM1, a novel metabolic marker deregulated in human malignancies. 致癌基因，2018年1月26日. doi: 10.1038/s41388-017-0051-9. [印前Epub]
Liu R, Wang X, Curtiss C, Landas S, Rong R, Sheikh MS, Huang Y. Monoglyceride lipase gene knockout in mice leads to increased incidence of lung adenocarcinoma. 细胞死亡病 . 2018年1月18日;9(2):36. doi: 10.1038/s41419-017-0188-z
Babbar M, Huang Y, Curtiss CM and Sheikh MS. CHTM1 regulates cancer cell sensitivity to metabolic stress via p38-AIF1 pathway. J. Exp. & Clin Cancer. Res. 2019, 38: 271.
Liu R Wang X, Curtiss C, Sheikh MS, Huang Y. 单甘油酯脂肪酶通过促进x -连锁细胞凋亡抑制蛋白的降解介导肿瘤抑制作用. Cell death Differ, 2020, May 6 , Doi: 10.1038/s41418-020-0549-5. 印刷前在线.
帕特尔H，谢赫MS，黄颖. ECRG2, a novel transcriptional target of p53, modulates cancer sensitivity to DNA damage. 细胞死亡病. 2020, Jul 17; 11(7):544. doi:10.1038/s41419-020-2728-
Khayam AU, Patel H, Faiola NA, Figueroa Milla AE, Dilshad E, Mirza B, Huang Y, Sheikh MS. Quinovic acid purified from medicinal plant Fagonia indica mediates anticancer effects via death receptor 5. Mol细胞生物化学. 2020年7月30日. doi: 10 - 1007 / s11010 - 020 - 03841 - 4. 印刷前在线.
谢赫MS，黄y. Antibody-drug conjugates for breast cancer treatment. 最近帕特抗癌药物的发现. 2022, 18(2): 108-113.